Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry

Thomas Millar, Ronald Knighton, Jo-Anne Chuck

    Research output: Chapter in Book / Conference PaperChapter

    2 Citations (Scopus)

    Abstract

    Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to become more economic in cost and time as it negates the need for a specific primary antibody. Methods are described to use exogenously added biotin to complex biological samples to demonstrate western blotting, dot blots, and immunohistochemistry. These methods can be used in biological science tertiary teaching laboratories to allow novices to gain skills in a risk-free environment to promote student motivation and engagement.
    Original languageEnglish
    Title of host publicationDetection of Blotted Proteins: Methods and Protocols
    EditorsBiji T. Kurien, R. Hal Schofield
    Place of PublicationU.S.
    PublisherHumana Press
    Pages151-164
    Number of pages14
    ISBN (Electronic)9781493927180
    ISBN (Print)9781493927173
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Western immunoblotting
    • dot blots
    • immunology
    • study and teaching (higher)

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