Abstract
Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to become more economic in cost and time as it negates the need for a specific primary antibody. Methods are described to use exogenously added biotin to complex biological samples to demonstrate western blotting, dot blots, and immunohistochemistry. These methods can be used in biological science tertiary teaching laboratories to allow novices to gain skills in a risk-free environment to promote student motivation and engagement.
Original language | English |
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Title of host publication | Detection of Blotted Proteins: Methods and Protocols |
Editors | Biji T. Kurien, R. Hal Schofield |
Place of Publication | U.S. |
Publisher | Humana Press |
Pages | 151-164 |
Number of pages | 14 |
ISBN (Electronic) | 9781493927180 |
ISBN (Print) | 9781493927173 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- Western immunoblotting
- dot blots
- immunology
- study and teaching (higher)