Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry

Thomas Millar; Ronald Knighton; Jo-Anne Chuck

doi:10.1007/978-1-4939-2718-0_16

Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry

Thomas Millar, Ronald Knighton, Jo-Anne Chuck

School of Science

Research output: Chapter in Book / Conference Paper › Chapter

2 Citations (Scopus)

Abstract

Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to become more economic in cost and time as it negates the need for a specific primary antibody. Methods are described to use exogenously added biotin to complex biological samples to demonstrate western blotting, dot blots, and immunohistochemistry. These methods can be used in biological science tertiary teaching laboratories to allow novices to gain skills in a risk-free environment to promote student motivation and engagement.

Original language	English
Title of host publication	Detection of Blotted Proteins: Methods and Protocols
Editors	Biji T. Kurien, R. Hal Schofield
Place of Publication	U.S.
Publisher	Humana Press
Pages	151-164
Number of pages	14
ISBN (Electronic)	9781493927180
ISBN (Print)	9781493927173
DOIs	https://doi.org/10.1007/978-1-4939-2718-0_16
Publication status	Published - 2015

Keywords

Western immunoblotting
dot blots
immunology
study and teaching (higher)

Access to Document

10.1007/978-1-4939-2718-0_16

Cite this

@inbook{b846ebe982a3484f86caf57b7006a1a8,

title = "Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry",

abstract = "Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to become more economic in cost and time as it negates the need for a specific primary antibody. Methods are described to use exogenously added biotin to complex biological samples to demonstrate western blotting, dot blots, and immunohistochemistry. These methods can be used in biological science tertiary teaching laboratories to allow novices to gain skills in a risk-free environment to promote student motivation and engagement.",

keywords = "Western immunoblotting, dot blots, immunology, study and teaching (higher)",

author = "Thomas Millar and Ronald Knighton and Jo-Anne Chuck",

year = "2015",

doi = "10.1007/978-1-4939-2718-0_16",

language = "English",

isbn = "9781493927173",

pages = "151--164",

editor = "Kurien, {Biji T.} and Schofield, {R. Hal}",

booktitle = "Detection of Blotted Proteins: Methods and Protocols",

publisher = "Humana Press",

}

Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry. / Millar, Thomas; Knighton, Ronald; Chuck, Jo-Anne.
Detection of Blotted Proteins: Methods and Protocols. ed. / Biji T. Kurien; R. Hal Schofield. U.S.: Humana Press, 2015. p. 151-164.

Research output: Chapter in Book / Conference Paper › Chapter

TY - CHAP

T1 - Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry

AU - Millar, Thomas

AU - Knighton, Ronald

AU - Chuck, Jo-Anne

PY - 2015

Y1 - 2015

N2 - Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to become more economic in cost and time as it negates the need for a specific primary antibody. Methods are described to use exogenously added biotin to complex biological samples to demonstrate western blotting, dot blots, and immunohistochemistry. These methods can be used in biological science tertiary teaching laboratories to allow novices to gain skills in a risk-free environment to promote student motivation and engagement.

AB - Using biotinylated targets for detection by enzyme-linked avidin allows immunodetection methods to become more economic in cost and time as it negates the need for a specific primary antibody. Methods are described to use exogenously added biotin to complex biological samples to demonstrate western blotting, dot blots, and immunohistochemistry. These methods can be used in biological science tertiary teaching laboratories to allow novices to gain skills in a risk-free environment to promote student motivation and engagement.

KW - Western immunoblotting

KW - dot blots

KW - immunology

KW - study and teaching (higher)

UR - http://handle.uws.edu.au:8081/1959.7/uws:30615

U2 - 10.1007/978-1-4939-2718-0_16

DO - 10.1007/978-1-4939-2718-0_16

M3 - Chapter

C2 - 26139263

SN - 9781493927173

SP - 151

EP - 164

BT - Detection of Blotted Proteins: Methods and Protocols

A2 - Kurien, Biji T.

A2 - Schofield, R. Hal

PB - Humana Press

CY - U.S.

ER -

Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots and immunohistochemistry

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this