TY - JOUR
T1 - Using curtain flow second-generation silica monoliths to improve separations at pressures less than 400 bar
AU - Soliven, A.
AU - Pravadali-Cekic, S.
AU - Foley, D.
AU - Pereira, L.
AU - Dennis, G. R.
AU - Cabrera, K.
AU - Ritchie, H.
AU - Edge, T.
AU - Shalliker, R. A.
PY - 2016
Y1 - 2016
N2 - The performance of a second-generation analytical-scale silica monolith utilizing the curtain flow (CF) column design was studied for the first time. The performance of the CF column was compared to a conventional monolith column under isocratic conditions, and the new column achieved a gain in theoretical plates by as much as 50%, with almost Gaussian bands being obtained (asymmetry factor of 1.02). Detection sensitivity increased by as much as 125% under optimal detection conditions. The sensitivity and performance was also chromatographically compared to a core-shell column under both gradient and isocratic elution for a model compound (polystyrene) and a natural product (coffee sample) with sensitivity gains of up to 365%. The second-generation monolithic CF column is an excellent tool that can be tuned to provide highly efficient, highly sensitive, high-throughput, and low volumetric detection analyses, using conventional HPLC systems.
AB - The performance of a second-generation analytical-scale silica monolith utilizing the curtain flow (CF) column design was studied for the first time. The performance of the CF column was compared to a conventional monolith column under isocratic conditions, and the new column achieved a gain in theoretical plates by as much as 50%, with almost Gaussian bands being obtained (asymmetry factor of 1.02). Detection sensitivity increased by as much as 125% under optimal detection conditions. The sensitivity and performance was also chromatographically compared to a core-shell column under both gradient and isocratic elution for a model compound (polystyrene) and a natural product (coffee sample) with sensitivity gains of up to 365%. The second-generation monolithic CF column is an excellent tool that can be tuned to provide highly efficient, highly sensitive, high-throughput, and low volumetric detection analyses, using conventional HPLC systems.
KW - active flow technology
KW - curtain flow chromatography
KW - high performance liquid chromatography
KW - silica monoliths
UR - http://handle.uws.edu.au:8081/1959.7/uws:34397
U2 - 10.1016/j.microc.2016.02.007
DO - 10.1016/j.microc.2016.02.007
M3 - Article
VL - 127
SP - 68
EP - 73
JO - Microchemical Journal
JF - Microchemical Journal
ER -