TY - JOUR
T1 - Using reaction flow chromatography for the analysis of amino acid : derivatisation with fluorescamine reagent
AU - Pravadali-Cekic, Sercan
AU - Jones, Andrew
AU - Kazarian, Artaches A.
AU - Paull, Brett
AU - Soliven, Arianne
AU - Ritchie, Harald
AU - Camenzuli, Michelle
AU - Dennis, Gary R.
AU - Shalliker, R. Andrew
PY - 2015
Y1 - 2015
N2 - Reaction flow (RF) chromatography with fluorescamine reagent and UV-vis detection was used for the analysis of amino acids. The performance advantage of RF chromatography was tested against the conventional post-column derivatisation (PCD) methods. The results of the study verified that greater sensitivity could be obtained using RF columns compared to conventional PCD methods. The reaction loops employed in the conventional PCD methods did not need to be used, which resulted in more efficient separations using RF columns. The selective detection of amino acids in this RF study reduced limits of detection (LOD) and limits of quantification (LOQ) by about 50% compared to conventional methods of PCD. The calibration data of the RF-PCD technique also yielded R2≥0.99 and %RSD in peak areas ranging from 3.0-8.5%. The RF method also enabled multiplexed detection since both the derivatised and non-derivatised flow streams could be analysed simultaneously.
AB - Reaction flow (RF) chromatography with fluorescamine reagent and UV-vis detection was used for the analysis of amino acids. The performance advantage of RF chromatography was tested against the conventional post-column derivatisation (PCD) methods. The results of the study verified that greater sensitivity could be obtained using RF columns compared to conventional PCD methods. The reaction loops employed in the conventional PCD methods did not need to be used, which resulted in more efficient separations using RF columns. The selective detection of amino acids in this RF study reduced limits of detection (LOD) and limits of quantification (LOQ) by about 50% compared to conventional methods of PCD. The calibration data of the RF-PCD technique also yielded R2≥0.99 and %RSD in peak areas ranging from 3.0-8.5%. The RF method also enabled multiplexed detection since both the derivatised and non-derivatised flow streams could be analysed simultaneously.
KW - amino acids
KW - chromatography
KW - chromatography columns
UR - http://handle.uws.edu.au:8081/1959.7/uws:30785
U2 - 10.1016/j.microc.2015.02.010
DO - 10.1016/j.microc.2015.02.010
M3 - Article
VL - 121
SP - 141
EP - 149
JO - Microchemical Journal
JF - Microchemical Journal
ER -