Using stereotactic brain atlases for small rodents and nonhuman primates for optrode array customization

Ronald W. Boutte, Sam Merlin, Brandon Griffiths, Trent Parry, Steve Blair

Research output: Chapter in Book / Conference PaperConference Paperpeer-review

Abstract

As the optogenetic field expands its need to target with high specificity only grows more crucial. This work will show a method for customizing soda-lime glass optrode arrays so that fine structures within the brains of small rodents and nonhuman primates can be optically interrogated below the outer cortical layer. An 8 × 6 array is customized for optrode length (400 μm ), optrode width (75 μm ), optrode pitch (400 μm ), backplane thickness (500 μm ), and overall form factor (3.45 mm × 2.65 mm ). The 400 μm long optrode is capable of illuminating the cortical Layer IV of rhesus macaque ( Macaca Fascicularis ) and the motor cortex of small mice ( Mus Musculus ).
Original languageEnglish
Title of host publicationProceedings of SPIE Conference on Progress in Biomedical Optics and Imaging, Vol. 10052: Optogenetics and Optical Manipulation, 28–29 January 2017, San Francisco, California, United States
PublisherSPIE
Number of pages5
ISBN (Print)9781510605459
DOIs
Publication statusPublished - 2017
EventSPIE Conference on Progress in Biomedical Optics and Imaging -
Duration: 28 Jan 2017 → …

Publication series

Name
ISSN (Print)1605-7422

Conference

ConferenceSPIE Conference on Progress in Biomedical Optics and Imaging
Period28/01/17 → …

Keywords

  • optogenetics
  • primates
  • rodents

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