TY - JOUR
T1 - Utilising retention correlation for the separation of oligostyrenes by coupled-column liquid chromatography
AU - Gray, Michael J.
AU - Dennis, Gary R.
AU - Slonecker, Patrick J.
AU - Shalliker, Ross A.
PY - 2005/5/6
Y1 - 2005/5/6
N2 - The separation of n = 2-5 n-butyloligostyrenes has been illustrated by reversed-phase reversed-phase (RP-RP) coupled-column liquid chromatography. The coupled-column separation has been achieved by use of a C 18 column with methanol as the mobile phase followed by a DiamondBond C 18 column with acetonitrile (ACN) mobile phase. The DiamondBond C 18 is a hybrid carbon clad zirconia (CCZ)-C 18 stationary phase. Unlike a C 18-carbon clad zirconia two-dimensional liquid chromatographic system, which is orthogonal, the C 18 and DiamondBond C 18 columns combination exhibit correlations based upon the molecular weight of n-butyloligostyrenes. Using an alternative strategy to two-dimensional liquid chromatography, the molecular weight dependence displayed by both the C 18 column and DiamondBond C 18 has been used to increase throughput or decrease analysis time in the analysis of the n- butyloligostyrenes. However, this is at the expense of a portion of the two-dimensional peak capacity displayed by the C 18-carbon clad zirconia system.
AB - The separation of n = 2-5 n-butyloligostyrenes has been illustrated by reversed-phase reversed-phase (RP-RP) coupled-column liquid chromatography. The coupled-column separation has been achieved by use of a C 18 column with methanol as the mobile phase followed by a DiamondBond C 18 column with acetonitrile (ACN) mobile phase. The DiamondBond C 18 is a hybrid carbon clad zirconia (CCZ)-C 18 stationary phase. Unlike a C 18-carbon clad zirconia two-dimensional liquid chromatographic system, which is orthogonal, the C 18 and DiamondBond C 18 columns combination exhibit correlations based upon the molecular weight of n-butyloligostyrenes. Using an alternative strategy to two-dimensional liquid chromatography, the molecular weight dependence displayed by both the C 18 column and DiamondBond C 18 has been used to increase throughput or decrease analysis time in the analysis of the n- butyloligostyrenes. However, this is at the expense of a portion of the two-dimensional peak capacity displayed by the C 18-carbon clad zirconia system.
KW - Chromatographic analysis
KW - Liquid chromatography
KW - Multidimensional chromatography
KW - Oligostyrene
KW - Separation (Technology)
UR - http://handle.uws.edu.au:8081/1959.7/10582
U2 - 10.1016/j.chroma.2004.10.071
DO - 10.1016/j.chroma.2004.10.071
M3 - Article
C2 - 15909499
SN - 0021-9673
VL - 1073
SP - 3
EP - 9
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -