Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A. Neville, Annabelle LeCordier, Helen Ziochos, Mathew J. Chater, Iain B. Gosbell, Michael W. Maley, Sebastiaan J. Van Hal

    Research output: Contribution to journalArticlepeer-review

    168 Citations (Scopus)

    Abstract

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.
    Original languageEnglish
    Pages (from-to)2980-2984
    Number of pages5
    JournalJournal of Clinical Microbiology
    Volume49
    Issue number8
    DOIs
    Publication statusPublished - 2011

    Keywords

    • bacterial diseases
    • enterobacter
    • escherichia coli
    • gram-positive bacteria
    • matrix-assisted laser desorption-ionization
    • micrococcus luteus
    • pseudomonas aeruginosa
    • salmonella
    • shigella
    • staphylococcus
    • streptococcus
    • time-of-flight mass spectrometry

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