Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A. Neville; Annabelle LeCordier; Helen Ziochos; Mathew J. Chater; Iain B. Gosbell; Michael W. Maley; Sebastiaan J. Van Hal

doi:10.1128/JCM.00431-11

Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A. Neville, Annabelle LeCordier, Helen Ziochos, Mathew J. Chater, Iain B. Gosbell, Michael W. Maley, Sebastiaan J. Van Hal

School of Medicine

Research output: Contribution to journal › Article › peer-review

173 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

Original language	English
Pages (from-to)	2980-2984
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00431-11
Publication status	Published - 2011

Keywords

bacterial diseases
enterobacter
escherichia coli
gram-positive bacteria
matrix-assisted laser desorption-ionization
micrococcus luteus
pseudomonas aeruginosa
salmonella
shigella
staphylococcus
streptococcus
time-of-flight mass spectrometry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JCM.00431-11

Cite this

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title = "Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification",

abstract = "Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.",

keywords = "bacterial diseases, enterobacter, escherichia coli, gram-positive bacteria, matrix-assisted laser desorption-ionization, micrococcus luteus, pseudomonas aeruginosa, salmonella, shigella, staphylococcus, streptococcus, time-of-flight mass spectrometry",

author = "Neville, {Stephen A.} and Annabelle LeCordier and Helen Ziochos and Chater, {Mathew J.} and Gosbell, {Iain B.} and Maley, {Michael W.} and {Van Hal}, {Sebastiaan J.}",

year = "2011",

doi = "10.1128/JCM.00431-11",

language = "English",

volume = "49",

pages = "2980--2984",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

AU - Neville, Stephen A.

AU - LeCordier, Annabelle

AU - Ziochos, Helen

AU - Chater, Mathew J.

AU - Gosbell, Iain B.

AU - Maley, Michael W.

AU - Van Hal, Sebastiaan J.

PY - 2011

Y1 - 2011

N2 - Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

AB - Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

KW - bacterial diseases

KW - enterobacter

KW - escherichia coli

KW - gram-positive bacteria

KW - matrix-assisted laser desorption-ionization

KW - micrococcus luteus

KW - pseudomonas aeruginosa

KW - salmonella

KW - shigella

KW - staphylococcus

KW - streptococcus

KW - time-of-flight mass spectrometry

UR - http://handle.uws.edu.au:8081/1959.7/525751

U2 - 10.1128/JCM.00431-11

DO - 10.1128/JCM.00431-11

M3 - Article

C2 - 21632894

SN - 0095-1137

VL - 49

SP - 2980

EP - 2984

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 8

ER -

Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Abstract

Keywords

UN SDGs

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