Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A. Neville; Annabelle LeCordier; Helen Ziochos; Mathew J. Chater; Iain B. Gosbell; Michael W. Maley; Sebastiaan J. Van Hal

doi:10.1128/JCM.00431-11

Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A. Neville
, Annabelle LeCordier
, Helen Ziochos
, Mathew J. Chater
, Iain B. Gosbell
, Michael W. Maley
, Sebastiaan J. Van Hal

School of Medicine

Research output: Contribution to journal › Article › peer-review

175 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

Original language	English
Pages (from-to)	2980-2984
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00431-11
Publication status	Published - 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

bacterial diseases
enterobacter
escherichia coli
gram-positive bacteria
matrix-assisted laser desorption-ionization
micrococcus luteus
pseudomonas aeruginosa
salmonella
shigella
staphylococcus
streptococcus
time-of-flight mass spectrometry

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10.1128/JCM.00431-11

Cite this

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title = "Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification",

abstract = "Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85\%) species and 2,681/2,781 (96\%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD\$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.",

keywords = "bacterial diseases, enterobacter, escherichia coli, gram-positive bacteria, matrix-assisted laser desorption-ionization, micrococcus luteus, pseudomonas aeruginosa, salmonella, shigella, staphylococcus, streptococcus, time-of-flight mass spectrometry",

author = "Neville, \{Stephen A.\} and Annabelle LeCordier and Helen Ziochos and Chater, \{Mathew J.\} and Gosbell, \{Iain B.\} and Maley, \{Michael W.\} and \{Van Hal\}, \{Sebastiaan J.\}",

year = "2011",

doi = "10.1128/JCM.00431-11",

language = "English",

volume = "49",

pages = "2980--2984",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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}

TY - JOUR

T1 - Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

AU - Neville, Stephen A.

AU - LeCordier, Annabelle

AU - Ziochos, Helen

AU - Chater, Mathew J.

AU - Gosbell, Iain B.

AU - Maley, Michael W.

AU - Van Hal, Sebastiaan J.

PY - 2011

Y1 - 2011

N2 - Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

AB - Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

KW - bacterial diseases

KW - enterobacter

KW - escherichia coli

KW - gram-positive bacteria

KW - matrix-assisted laser desorption-ionization

KW - micrococcus luteus

KW - pseudomonas aeruginosa

KW - salmonella

KW - shigella

KW - staphylococcus

KW - streptococcus

KW - time-of-flight mass spectrometry

UR - http://handle.uws.edu.au:8081/1959.7/525751

U2 - 10.1128/JCM.00431-11

DO - 10.1128/JCM.00431-11

M3 - Article

C2 - 21632894

SN - 0095-1137

VL - 49

SP - 2980

EP - 2984

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 8

ER -

Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Abstract

UN SDGs

Keywords

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