Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas

D. J. E. McKnight; J. Wong-Bajracharya; Efenaide B. Okoh; F. Snijders; F. Lidbetter; J. Webster; M. Haughton; A. E. Darling; S. P. Djordjevic; D. R. Bogema; T. A. Chapman

doi:10.1099/ijsem.0.006294

Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas

D. J. E. McKnight, J. Wong-Bajracharya, Efenaide B. Okoh, F. Snijders, F. Lidbetter, J. Webster, M. Haughton, A. E. Darling, S. P. Djordjevic, D. R. Bogema, T. A. Chapman

Western Sydney University

Research output: Contribution to journal › Article › peer-review

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Abstract

Five bacterial isolates were isolated from Fragaria × ananassa in 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these isolates represented members of the genus Xanthomonas. To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates represented members of the genus Xanthomonas but did not allow them to be classified with respect to species. Genome relatedness indices and the results of extensive phylogenetic analysis confirmed that the isolates were members of the genus Xanthomonas and represented a novel species. On the basis the minimal presence of virulence-associated factors typically found in genomes of members of the genus Xanthomonas, we suggest that these isolates are non-pathogenic. This conclusion was supported by the results of a pathogenicity assay. On the basis of these findings, we propose the name Xanthomonas rydalmerensis, with DAR 34855T = ICMP 24941 as the type strain.

Original language	English
Article number	006294
Number of pages	12
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	74
Issue number	3
DOIs	https://doi.org/10.1099/ijsem.0.006294
Publication status	Published - 1 Mar 2024

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Publisher Copyright:
© 2024 CROWN.

Open Access - Access Right Statement

This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/deed.en).

Keywords

bioinformatics
biosecurity
nonpathogenic
phylogenetics
Xanthomonas

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McKnight, D. J. E., Wong-Bajracharya, J., Okoh, E. B., Snijders, F., Lidbetter, F., Webster, J., Haughton, M., Darling, A. E., Djordjevic, S. P., Bogema, D. R., & Chapman, T. A. (2024). Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas. International Journal of Systematic and Evolutionary Microbiology, 74(3), Article 006294. https://doi.org/10.1099/ijsem.0.006294

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title = "Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas",

abstract = "Five bacterial isolates were isolated from Fragaria × ananassa in 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these isolates represented members of the genus Xanthomonas. To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates represented members of the genus Xanthomonas but did not allow them to be classified with respect to species. Genome relatedness indices and the results of extensive phylogenetic analysis confirmed that the isolates were members of the genus Xanthomonas and represented a novel species. On the basis the minimal presence of virulence-associated factors typically found in genomes of members of the genus Xanthomonas, we suggest that these isolates are non-pathogenic. This conclusion was supported by the results of a pathogenicity assay. On the basis of these findings, we propose the name Xanthomonas rydalmerensis, with DAR 34855T = ICMP 24941 as the type strain.",

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author = "McKnight, {D. J. E.} and J. Wong-Bajracharya and Okoh, {Efenaide B.} and F. Snijders and F. Lidbetter and J. Webster and M. Haughton and Darling, {A. E.} and Djordjevic, {S. P.} and Bogema, {D. R.} and Chapman, {T. A.}",

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McKnight, DJE, Wong-Bajracharya, J, Okoh, EB, Snijders, F, Lidbetter, F, Webster, J, Haughton, M, Darling, AE, Djordjevic, SP, Bogema, DR & Chapman, TA 2024, 'Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas', International Journal of Systematic and Evolutionary Microbiology, vol. 74, no. 3, 006294. https://doi.org/10.1099/ijsem.0.006294

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T1 - Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas

AU - McKnight, D. J. E.

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AU - Okoh, Efenaide B.

AU - Snijders, F.

AU - Lidbetter, F.

AU - Webster, J.

AU - Haughton, M.

AU - Darling, A. E.

AU - Djordjevic, S. P.

AU - Bogema, D. R.

AU - Chapman, T. A.

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N2 - Five bacterial isolates were isolated from Fragaria × ananassa in 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these isolates represented members of the genus Xanthomonas. To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates represented members of the genus Xanthomonas but did not allow them to be classified with respect to species. Genome relatedness indices and the results of extensive phylogenetic analysis confirmed that the isolates were members of the genus Xanthomonas and represented a novel species. On the basis the minimal presence of virulence-associated factors typically found in genomes of members of the genus Xanthomonas, we suggest that these isolates are non-pathogenic. This conclusion was supported by the results of a pathogenicity assay. On the basis of these findings, we propose the name Xanthomonas rydalmerensis, with DAR 34855T = ICMP 24941 as the type strain.

AB - Five bacterial isolates were isolated from Fragaria × ananassa in 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these isolates represented members of the genus Xanthomonas. To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates represented members of the genus Xanthomonas but did not allow them to be classified with respect to species. Genome relatedness indices and the results of extensive phylogenetic analysis confirmed that the isolates were members of the genus Xanthomonas and represented a novel species. On the basis the minimal presence of virulence-associated factors typically found in genomes of members of the genus Xanthomonas, we suggest that these isolates are non-pathogenic. This conclusion was supported by the results of a pathogenicity assay. On the basis of these findings, we propose the name Xanthomonas rydalmerensis, with DAR 34855T = ICMP 24941 as the type strain.

KW - bioinformatics

KW - biosecurity

KW - nonpathogenic

KW - phylogenetics

KW - Xanthomonas

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VL - 74

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

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M1 - 006294

ER -

Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of group 1 Xanthomonas

Abstract

Bibliographical note

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Keywords

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