TY - JOUR
T1 - Yellow lupin (Lupinus luteus L.) polysaccharides : antioxidant, immunomodulatory and prebiotic activities and their structural characterisation
AU - Thambiraj, Solomon Rajesh
AU - Phillips, Michael
AU - Koyyalamudi, Sundar Rao
AU - Reddy, Narsimha
PY - 2018
Y1 - 2018
N2 - Yellow lupin polysaccharides (YLP-1, YLP-2 and YLP-3) were isolated from the whole seeds of Lupinus luteus L. Their antioxidant activities were evaluated by ABTS+• and hydroxyl radical scavenging, and Fe2+ chelating assays. Immunostimulatory activities were measured by their ability to activate macrophages to produce TNF-α and NO. Four strains of probiotic bacteria were used to measure their prebiotic activities. YLP-2 with largest galactose content displayed the best activity amongst the three isolated polysacchardies. NMR and FT-IR spectroscopic methods have revealed that YLPs contain galactans and galactomannans which are linked with β- (1, 4) glycosidic bond in the main chain. The side chain Galp unit of galactomannan is connected to the main chain Manp by α - (1, 6) linkage. The results presented in this paper strongly suggest that YLPs display significant antioxidant, immunostimulatory and prebiotic activities and hence hold great potential as nutraceutical and functional agents.
AB - Yellow lupin polysaccharides (YLP-1, YLP-2 and YLP-3) were isolated from the whole seeds of Lupinus luteus L. Their antioxidant activities were evaluated by ABTS+• and hydroxyl radical scavenging, and Fe2+ chelating assays. Immunostimulatory activities were measured by their ability to activate macrophages to produce TNF-α and NO. Four strains of probiotic bacteria were used to measure their prebiotic activities. YLP-2 with largest galactose content displayed the best activity amongst the three isolated polysacchardies. NMR and FT-IR spectroscopic methods have revealed that YLPs contain galactans and galactomannans which are linked with β- (1, 4) glycosidic bond in the main chain. The side chain Galp unit of galactomannan is connected to the main chain Manp by α - (1, 6) linkage. The results presented in this paper strongly suggest that YLPs display significant antioxidant, immunostimulatory and prebiotic activities and hence hold great potential as nutraceutical and functional agents.
KW - antioxidants
KW - lupines
KW - polysaccharides
UR - http://handle.westernsydney.edu.au:8081/1959.7/uws:45816
U2 - 10.1016/j.foodchem.2018.02.111
DO - 10.1016/j.foodchem.2018.02.111
M3 - Article
SN - 0308-8146
VL - 267
SP - 319
EP - 328
JO - Food Chemistry
JF - Food Chemistry
ER -