Understanding the intracellular dynamics of endogenous ribonuclein acid species in living cells

Abstract

Overall this thesis presents the application of different methods to assess natural endogenous RNA dynamics. The developed models to identify the properties of the labelled transcripts in live cells may be applied to fields that assess the response of living cells to RNA therapeutics, cancer pathology and RNA virology introduction. In addition, it aims to minimise the gaps in current knowledge of transcript properties while in living cells, including how the species are mobilised throughout live and mobile cells, how to identify the number of different RNA subspecies when labelled by a single probe as well as the development of methods which potentially enable the real time monitoring of endogenous transcript responses upon the introduction of a stimuli.

Date of Award	2012
Original language	English