Uptake of trans-resveratrol by yeast cells

Abstract

This study investigates the uptake of the stilbene trans-resveratrol into Saccharomyces cerevisiae yeast cells. Stilbenes are used in ageing studies and are believed to increase longevity, have strong antioxidant activity and show protective effect against atherosclerosis, coronary heart disease, postmenopausal problems, cancer and a broad spectrum of degenerative diseases. S. cerevisiae yeast has been used for thousands of years and is widely used today in genetics and cell biology research as it genome is known. In this study, S.cerevisiae yeast cell is used, for the first time, as a model eukaryotic system to determine the uptake of trans-resveratrol in all eukaryotes. Synthetic yeast growth media containing 0.5% and 2% glucose were used to determine the effect of calorie variation on trans-resveratrol uptake rate. The yeast cells were exposed to 2.5, 5.0, 10, 50 and 100 M (or 0.57, 1.14, 2.28, 11.41 and 22.82 g/mL) trans-resveratrol in media and incubated for 1, 24, 96, 240 and 480 h. After incubation, the samples were centrifuged and the supernatant cleaned up by C18 solid-phase extraction before analysis of trans-resveratrol by high performance liquid chromatography with photodiode array detection (HPLC-PDA) at 307 nm. The yeast cells were lysed by ultrasonication in a 2:1 (v/v) chloroform-methanol mixture, then dried and weighed before analysis as for the supernatant. The LOQ of trans-resveratrol in the matrices used in this study was found to be 0.57 g/mL and the mean recovery was 88.1 5.6 %. Trans-resveratrol uptake increased with increasing concentration in the media. Uptake in the calorie restricted medium (0.5% glucose) was found to steadily increase over the study time period while in the non-calorie restricted medium (2% glucose), uptake peaked at 10 days. A DPPH scavenging activity assay of the compound showed that it had high antioxidant activity.

Date of Award	2012
Original language	English