Using proteomics and CRISPR to identify key host regulatory proteins involved in host-parasite interactions following the attachment of Trichomonas vaginalis

Abstract

Trichomonas vaginalis is an extracellular protozoan parasite responsible for the most common non-viral sexually transmitted infection in the world, infecting an estimated 170 million people per annum. The current model for T. vaginalis pathogenesis is still not clearly understood due to the number of mechanisms the parasite utilizes during infection. This project will focus on protein changes within host cells following interaction with T. vaginalis. Specifically, the changes in protein expression within host cells post-parasite attachment. Host proteins will then be analysed using SWATH analysis to identify the proteins and ultimately the genes that encode these proteins. Using DNA editing technologies (CRISPR/Cas9), genes found to be upregulated following interaction will be targeted for knockdown. This can potentially result in the identification of transmission blocking candidates. Results showed the need for further analysis in identifying targets for knockdown.

Date of Award	2017
Original language	English